{"id":690,"date":"2012-11-10T15:54:34","date_gmt":"2012-11-10T14:54:34","guid":{"rendered":"http:\/\/saber.comule.com\/?p=690"},"modified":"2020-11-12T19:01:06","modified_gmt":"2020-11-12T18:01:06","slug":"shotgun-sequencing","status":"publish","type":"post","link":"http:\/\/saber-benmimoun.com\/?p=690","title":{"rendered":"Shotgun sequencing"},"content":{"rendered":"<p>For sequencing long DNA, even an entire chromosome.<\/p>\n<p>Not reliable in case of DNA with lots of repetitions (need to use the sequencing method based on BACs).<\/p>\n<p><strong>Steps<\/strong><\/p>\n<ul>\n<li>Random DNA fragmenation (restriction enzymes or physical fragmenation)<\/li>\n<li>Sequencing of individual fragments.<\/li>\n<li>Assembly of the\u00a0fragments sequences by computer on the basis of their overlapping regions.<\/li>\n<\/ul>\n<p><a href=\"http:\/\/saber.comule.com\/wp-content\/uploads\/2012\/11\/Nouveau-Image-bitmap.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-691 aligncenter\" title=\"\" src=\"http:\/\/saber.comule.com\/wp-content\/uploads\/2012\/11\/Nouveau-Image-bitmap-300x181.jpg\" alt=\"\" width=\"300\" height=\"181\" srcset=\"http:\/\/saber-benmimoun.com\/wp-content\/uploads\/2012\/11\/Nouveau-Image-bitmap-300x181.jpg 300w, http:\/\/saber-benmimoun.com\/wp-content\/uploads\/2012\/11\/Nouveau-Image-bitmap-1024x619.jpg 1024w, http:\/\/saber-benmimoun.com\/wp-content\/uploads\/2012\/11\/Nouveau-Image-bitmap.jpg 1872w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>For sequencing long DNA, even an entire chromosome. Not reliable in case of DNA with lots of repetitions (need to use the sequencing method based on BACs). Steps Random DNA fragmenation (restriction enzymes or physical fragmenation) Sequencing of individual fragments. Assembly of the\u00a0fragments sequences by computer on the basis of their overlapping regions.<\/p>\n","protected":false},"author":1,"featured_media":691,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[7],"tags":[],"class_list":["post-690","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biologie","category-7","description-off"],"_links":{"self":[{"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/posts\/690","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=690"}],"version-history":[{"count":7,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/posts\/690\/revisions"}],"predecessor-version":[{"id":2952,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/posts\/690\/revisions\/2952"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=\/wp\/v2\/media\/691"}],"wp:attachment":[{"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=690"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=690"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/saber-benmimoun.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=690"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}